Strychnos colubrina L. (Loganiaceae) is being used in traditional medicine for a variety of ailments. Four successive standards and four crude extract of its barks were subjected for in-vitro antioxidant activity using p-NDA hydroxyl scavenging activity. The successive methanol extract have shown potent antioxidant activity in p-NDA method with 65±0.001, 72±0.008, 59±0.001, 51±0.002 (mean±S.E.M) for 10 µg/ml, 20 µg/ml, 40 µg/ml and 80 µg/ml, respectively. The crude methanol has shown moderate antioxidant activity in scavenging of hydroxyl radical by p-NDA method. All the concentration prepared was paying dose dependent antioxidant activity.
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